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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: The Effect of Cholesterol Efflux on Endothelial Dysfunction Caused by Oxidative Stress
doi: 10.3390/ijms24065939
Figure Lengend Snippet: H 2 O 2 can promote cholesterol accumulation by oxidative stress. ( A , B ) The protein levels of LXRα, LXRβ, HMGCR, ABCA1, and ABCG1 were measured by a Western blot analysis. ( C ) The accumulation of ER cholesterol after filipin staining was visualized under a fluorescence microscope (magnification 5×). n = 3, * p < 0.05, and ** p < 0.001 vs. the C group (treated by PBS). C, control group; H 2 O 2 , cells treated with H 2 O 2 (500 μmol/L) for 24 h. The scale of the pictures was 100 μm.
Article Snippet: The antibodies are as follows: MCP-1 (WL02966, Wanlei, Shenyang, China), 1:500; CD106/VCAM-1 (WL02474, Wanlei, Shenyang, China), 1:500; GRP78 (66574-1-Ig, Proteintech, Hubei, China), 1:10000; GAPDH (HRP-60004, Proteintech, Hubei, China), 1:40000; β-catenin (17565-1-AP, Proteintech, Hubei, China), 1:4000; ICAM-1 (10831-1-AP, Proteintech, Hubei, China), 1:1000; ABCA1 (D155299, BBI, Shanghai, China), 1:500; ABCG1 (AP6529A, Abgent, San Diego, USA), 1:500; LXRα (D198471, BBI, Shanghai, China), 1:2000;
Techniques: Western Blot, Staining, Fluorescence, Microscopy, Control